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pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

Plasmid

#84253

Purpose

Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJL580

Plasmid

#78185

Purpose

Ce rpy-1 expression in worms

Depositor

Junho Lee

Article

Nam et al J Biol Chem. 2009 Mar 20;284(12):8195-20

Insert

rpy-1 (rpy-1 Nematode)

Expression

Worm

Promoter

rpy-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pSCKtheoRaj12

Plasmid

#69942

Purpose

pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj12,cisRaj12,sfGFP

Use

Synthetic Biology

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj11

Plasmid

#69939

Purpose

pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj11,cisRaj11,sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSCKbreak12

Plasmid

#69938

Purpose

pSTC2,cisRaj12-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

CisRaj12-sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12-o11

Plasmid

#69941

Purpose

pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj12,cisRaj11,sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

Plasmid

#69222

Purpose

Expresses R1333S mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Serine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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Wnt10B Non-STOP

Plasmid

#34748

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (Wnt10b Human)

Use

Gateway entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt9B Non-STOP

Plasmid

#35901

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Use

Entry vector

Mutation

SNP Met106-->Thr

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt11 Non-STOP

Plasmid

#35903

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt16 Non-STOP

Plasmid

#35904

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt16 (WNT16 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt2B2 STOP

Plasmid

#35870

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2B2 (WNT2B Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt2 Non-STOP

Plasmid

#35888

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Use

Entry vector

Mutation

Silent Mutation G (816) --> A

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt3 Non-STOP

Plasmid

#35891

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt5B Non-STOP

Plasmid

#35894

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt6 Non-STOP

Plasmid

#35895

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Entry vector

Mutation

Silent Mutation G (567) --> A

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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