We narrowed to 14,291 results for: SHI

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pHLmMBP-11

Plasmid

#72349

Purpose

Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-mRipk3

Plasmid

#78805

Purpose

To overexpress mRipk3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (Ripk3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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p4947 pcDNA4c hBrd4 full-length

Plasmid

#14441

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe puro Myr HA PIK3CA

Plasmid

#12523

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA Myr (PIK3CA Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele; I14…

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

Plasmid

#176541

Purpose

human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB omegaRNA scaffold

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Etv6

Plasmid

#131599

Purpose

expression of mouse Etv6 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Etv6 (Etv6 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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TIRAP-GFP

Plasmid

#52739

Purpose

Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

Depositor

Jonathan Kagan

Article

Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

Insert

Toll/IL-1 Receptor adaptor protein (TIRAP Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pBP-split-intein-Gal4[21-881]-C-int

Plasmid

#199202

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/hArf4(T31N)-HA

Plasmid

#79405

Purpose

Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(Q71L)-HA

Plasmid

#79404

Purpose

Expresses C-terminally HA-tagged Arf4(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Mutation

Gln 71 Leu

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-HA

Plasmid

#79403

Purpose

Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1hSIRT3_H248Y_HA

Plasmid

#24492

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)

Tags

HA

Expression

Mammalian

Mutation

H248Y

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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NO MS-Sensor (35)

Plasmid

#199783

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO MS-Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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