We narrowed to 10,808 results for: T7

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pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066

Plasmid

#175587

Purpose

in vitro transcription of wt RBM8A 3'UTR

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A-3'UTR AluY-Sz WT

Use

In vitro transcription

Promoter

T7

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-E285K-N268D

Plasmid

#35089

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His and Thrombin cleavage site

Expression

Bacterial

Mutation

Contains aa 1-393 with N269D and E285K mutations

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only

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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

Plasmid

#99342

Purpose

Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

Depositor

Peter Wright

Article

Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

Inserts

STAT1 (710-750) (STAT1 Human)
CBP (1764-1855)

Tags

His6GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-kan

Plasmid

#108706

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

Plasmid

#123346

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-CKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRD398

Plasmid

#163105

Purpose

Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mCherry_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZDeltaAL

Plasmid

#99551

Purpose

Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Mutation

replaced 1557-1618 with SGGSG

Promoter

T7

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R49A)

Plasmid

#112726

Purpose

Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R49A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R49A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

Plasmid

#101231

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

Promoter

T7

Available Since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-GFP-FLARE-AKAR

Plasmid

#123332

Purpose

Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

GFP-GFP-FLARE-AKAR

Tags

6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

Plasmid

#173761

Purpose

Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

Depositor

Peter Wright

Article

Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-plus-NLS-6xHis

Plasmid

#207381

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, Q695A, Q926A, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ctdnep1_Cter

Plasmid

#196522

Purpose

Entry vector for Gateway with Ctdnep1_Cter

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-Cter (CTDNEP1 Human)

Expression

Bacterial

Mutation

No transmembrane domain

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Ctdnep1

Plasmid

#196520

Purpose

Entry vector for Gateway with Ctdnep1

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-N1 (CTDNEP1 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ctdnep1_D67E-2xFlag

Plasmid

#196519

Purpose

Expression of Ctdnep1_D67E-2xFlag

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67E-2xFlag (CTDNEP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead)

Promoter

CMV promoter; T7 promoter

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+CeGFP-Ctdnep1WTsiR

Plasmid

#196517

Purpose

Expression of Ctdnep1WT-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1WTsiR (CTDNEP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-EPHX1

Plasmid

#53114

Purpose

In vitro translation of microsomal epoxide hydrolase

Depositor

Xu Lin

Article

Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

Insert

EPHX1 (EPHX1 Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only