166,819 results

1. pAAV.GFA104.PI.eGFP.WPRE.bGH (AAV5)

   Viral Prep

   #100896-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.GFA104.PI.eGFP.WPRE.bGH (#100896). In addition to the viral particles, you will also receive purified pAAV.GFA104.PI.eGFP.WPRE.bGH plasmid DNA. GFA104-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Philip Haydon

   Article

   Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi

   Promoter

   GFA104 (GFAP promoter)

   Tags

   EGFP

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pSBbi G418R mbIL21

   Plasmid

   #194329

   Purpose

   Consitiutive mammalian expression of membrane bound human IL-21

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   mbIL-21 (IL21 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

   Plasmid

   #67974

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMD2.G-K47Q/R354Q

   Plasmid

   #207323

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q/R354Q

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MS2-HA-HshnRNPH1_Z

   Plasmid

   #148120

   Purpose

   Mammalian Expression of HshnRNPH1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPH1 (HNRNPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+DPP6

   Plasmid

   #117272

   Purpose

   Encodes human DPP6 variant 1

   Depositor

   Mark Cannell

   Article

   Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

   Insert

   DPP6 (DPP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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