Addgene

We narrowed to 1,081 results for: APK

Showing: 921 - 940 of 1081 results

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pLX302-MKP5-V5 puro

Plasmid

#87770

Purpose

Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP5 (DUSP10 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-p18G2A-HA

Plasmid

#42327

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 G2A (LAMTOR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

G2A

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA-HBXIP

Plasmid

#42328

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
PCRT7 tetR pLtetO MBP-MEK1 XX Amp

Plasmid

#53225

Purpose

human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

Depositor

Jesse Rinehart

Insert

MEK1 (MAP2K1 Human)

Use

Phosphoprotein synthesis

Tags

HIS and MBP

Expression

Bacterial

Mutation

Codon optimized for E.coli, both codons for Ser21…

Promoter

pLtetO

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK4alpha WT

Plasmid

#12187

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCMV5 MEKK4alpha KD

Plasmid

#12188

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K1361M

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pLX302-MKP7-V5 puro

Plasmid

#87771

Purpose

Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP7 (DUSP16 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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782 pMEActMEK

Plasmid

#31166

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

MEK (MAP2K1 Human)

Use

Entry vector

Tags

Expression

Mutation

Ser218Glu Ser222Asp; activated MEKK

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pMT2-MEKK3-K392A

Plasmid

#21585

Depositor

John Kyriakis

Article

Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

Insert

mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)

Use

Tags

Expression

Mammalian

Mutation

Lysine 392 to Arginine

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
L1

Plasmid

#21200

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

Mek2 K101A fused to ER*, catalytically inactive

Promoter

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pGL4_EGR1p-BC1484-luc2

Plasmid

#227138

Purpose

Barcoded assay, MAPK sensor; barcode BC1484

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 1484 and a luciferase reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC0136-luc2

Plasmid

#227137

Purpose

Barcoded assay, MAPK sensor; barcode BC0136

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0136 and a luciferase reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC0132-luc2

Plasmid

#227136

Purpose

Barcoded assay, MAPK sensor; barcode BC0132

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0132 and a luciferase reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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TurboID-MEK1

Plasmid

#226169

Purpose

Used to transiently express a TurboID fusion of the protein kinase MEK1.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-MEK1 (MAP2K1 Human)

Use

Tags

Myc-TurboID

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET-SOMA-T679A

Plasmid

#118936

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b(C631S, C643S and C699T)

Plasmid

#52128

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

C631S, C643S and C699T

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 T7 Jip1b(471-707)

Plasmid

#52428

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-470

Promoter

Available since

April 29, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b(282-707)

Plasmid

#52126

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-281

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 Jip1(1-470)

Plasmid

#51724

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted 471-660

Promoter

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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