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peft-3::cas-9::tbb-2 3'UTR

Plasmid

#48960

Purpose

this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Insert

cas9

Use

CRISPR

Promoter

eef-1A.1 (eft-3)

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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CH00X

Plasmid

#73517

Purpose

TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::TagRFP-T::PH::tbb-2

Tags

TagRFP-T

Promoter

mes-5

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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JH04

Plasmid

#73513

Purpose

mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mRuby2::PH::tbb-2

Tags

mRuby2

Promoter

mex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

Plasmid

#159796

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Prpl-3 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

Plasmid

#159896

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Tags

V5; mCherry

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EFF-1::V5

Plasmid

#132961

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

EFF-1::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Expression

Bacterial

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPK719

Plasmid

#38149

Depositor

Patricia Kuwabara

Insert

cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)

Use

Recombineering

Expression

Worm

Promoter

unc-119

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRG02

Plasmid

#44196

Depositor

Denis Dupuy

Insert

Neo

Expression

Worm

Promoter

rps-27

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his24::tagBFP2

Plasmid

#124344

Purpose

Nuclear Localized Pan-Neuronal tagBFP2

Depositor

Andrew Leifer

Insert

rab3, tagBFP2 (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKS2301

Plasmid

#37834

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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(p)odr-3::GFP::EGL-4

Plasmid

#100897

Purpose

GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

Depositor

Noelle L'Etoile

Article

Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

Insert

56-2741: odr-3 promoter

Expression

Worm

Promoter

odr-3

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Tags

GFP

Expression

Worm

Promoter

meg-3

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pRB1083

Plasmid

#71480

Purpose

Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VRER Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eft-3 (eef-1A.1)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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