Skip to main content
Addgene
Showing: 921 - 940 of 2276 results

  2. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

    Enlarge

  3. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73223-plasmid-map-sequence-id-135055

    Enlarge

  4. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73220-plasmid-map-sequence-id-134711

    Enlarge

  5. pICSL70004

    Plasmid
    #50334
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
    Use
    Plant expression
    Tags
    Expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50334-plasmid-map-sequence-id-75091

    Enlarge

  6. pGWB402SC

    Plasmid
    #224552
    Purpose
    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.
    Depositor
    Paul Schulze-Lefert
    Article
    Lawson et al bioRxiv 2024.07.14.603419
    Type
    Empty backbone
    Use
    Tags
    Twin-Strep-HA
    Expression
    Plant
    Mutation
    Promoter
    2 X 35S
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    224552-plasmid-map-sequence-id-444737

    Enlarge

  11. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

    Enlarge

  13. pOPS0382

    Plasmid
    #133232
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133232-plasmid-map-sequence-id-287534

    Enlarge

  14. pOPS0378

    Plasmid
    #133229
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133229-plasmid-map-sequence-id-276542

    Enlarge

  16. pOPS0380

    Plasmid
    #133230
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133230-plasmid-map-sequence-id-278559

    Enlarge

Showing: 921 - 940 of 2276 results