We narrowed to 1,166 results for: ncl

1. pTH442-SUP45-K176T

   Plasmid

   #29385

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K176T mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pTH444-SUP45-G181A

   Plasmid

   #29387

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   G181A mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pTH374-SUP45-R62A

   Plasmid

   #29391

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R62A mutant gene including genomic sequence 1432 …

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pTH356-SUP45-T295A

   Plasmid

   #29389

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T295A mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pTH393-SUP45-T357A

   Plasmid

   #29392

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T357A mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pTH357-SUP45-T388A

   Plasmid

   #29393

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T388A mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pTH363-SUP45-F401Y

   Plasmid

   #29394

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F401Y mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pTH364-SUP45-Y410F

   Plasmid

   #29395

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Y410F mutant gene including genomic sequence 1432…

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pET30-GBF-E6

   Plasmid

   #21696

   Depositor

   James Baleja

   Article

   Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

   Insert

   protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
   

   Use

   Tags

   Protein G B1 domain

   Expression

   Bacterial

   Mutation

   GB1 (1-56aa) fusion tag with mutations at three p…

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. DCT.VV.Orf217

    Plasmid

    #12905

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf217
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

11. DCT.VV.Orf219

    Plasmid

    #12907

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf219
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    U6

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-LC3

    Plasmid

    #21073

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLL3.7

    Plasmid

    #11795

    Purpose

    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

    Depositor

    Luk Parijs

    Article

    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. delCMV-mCherry-3x-p67phox-GBD

    Plasmid

    #214141

    Purpose

    Rac activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xp67phox-GBD (NCF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDest17_MAX

    Plasmid

    #234044

    Purpose

    Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

    Depositor

    Tanja Mittag

    Article

    Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

    Insert

    MAX (MAX Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEM-Cas9HF1-recA56

    Plasmid

    #89962

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet and proD

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-FLuc

    Plasmid

    #83281

    Purpose

    recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19_CoV-2_linker

    Plasmid

    #211731

    Purpose

    Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

    Depositor

    Thomas Klimkait

    Article

    Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

    Insert

    Linker fragment SARS-CoV-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid

    #194328

    Purpose

    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

    Depositor

    Alexander McLellan

    Article

    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Tet-responsive promotor related mutations: G72V i…

    Promoter

    Tight tet-responsive promotor

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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