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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,195 results for: sgRNA

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Showing: 921 - 940 of 9195 results

  2. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

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  3. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid
    #140205
    Purpose
    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    mCherry
    sgRNA E1
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Mutation
    G174D
    Promoter
    PelcA and U3
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140205-plasmid-map-sequence-id-310180

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  5. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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  6. pKLV-gRNA-CTBP2-MGMT_1

    Plasmid
    #136419
    Purpose
    Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136419-plasmid-map-sequence-id-271761

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Showing: 921 - 940 of 9195 results