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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,724 results for: ace

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Showing: 9381 - 9400 of 20724 results

  1. pINDUCER20_mStrawberry_Atg4BC74A

    Plasmid
    #161735
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161735-plasmid-map-sequence-id-320115

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  3. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  4. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  5. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196062-plasmid-map-sequence-id-385881

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  6. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  7. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  9. pLenti-rInsp-hIns-GLuc

    Plasmid
    #89927
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    410bp rat insulin II promoter
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    89927-plasmid-map-sequence-id-255126

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Showing: 9381 - 9400 of 20724 results