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FB003

Plasmid

#119677

Purpose

Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJ207 Delta-cpc-KanR

Plasmid

#51468

Purpose

Deletes the cpc operon in Synechocystis and confers kanamycin resistance

Depositor

Anastasios Melis

Article

Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

Insert

Replacing the cpc operon with kanamycin resistance

Use

Synthetic Biology

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FAM149A

Plasmid

#107510

Purpose

Expressing FAM149a, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FAM149A (FAM149A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgRBFOX1-2098

Plasmid

#115452

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2098 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgRBFOX1-2083

Plasmid

#115451

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2083 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC

Plasmid

#99649

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

10x His tag, Poly arginine linker, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC(Q120L)

Plasmid

#99701

Purpose

Bacterial expression of RagA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG159-foxD

Plasmid

#99068

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea foxD

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

foxD in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup88-HindIII

Plasmid

#87329

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [2_n-1] (GB1206)

Plasmid

#75407

Purpose

tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [2_n-1]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN (delta)N (aa1-117 deleted)

Plasmid

#73382

Purpose

Expresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Deleted N-terminal 1-117 aa from XPLN protein

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa1-125 (N125)

Plasmid

#73378

Purpose

Expression of the N-terminal region of XPLN (amino acids 1–125, designated N125)

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses N terminal amino acid 1-125 of XPLN (ar…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLentiX2-PURO-shDIXDC1-Ms-57

Plasmid

#61232

Purpose

Lentivirus expressing shRNA to mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

Dixdc1 (Dixdc1 Mouse)

Use

Lentiviral

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pBabe-Neuroserpin oloop

Plasmid

#58260

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC I317D

Plasmid

#47367

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, I317D

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
BC-GPRC

Plasmid

#44199

Depositor

Lei Xu

Insert

human GPR56 C-terminal fragment (ADGRG1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

C-terminal fragment of GPR56 starting at amino a…

Promoter

LTR

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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