We narrowed to 28,294 results for: cmv promoter
-
Plasmid#175494PurposeTotal RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.DepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pMH0004
Plasmid#174087PurposeUCOE-SFFV-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralPromoterSFFVAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_6P-D614G
Plasmid#172734PurposeExpression vector for SARS-CoV-2 (HexaPro-D614G) spike used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
Tags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable SinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD-DBD
Plasmid#171236PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Beta
Plasmid#171269PurposeMammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunitDepositorInsert3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (WT)
Plasmid#169720PurposeMammalian Expression of wild-type mouse SPOPDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 PE2*
Plasmid#169851PurposeMammalian Expression, sauCas9 based prime editorDepositorInsertSaCas9-N580A-M-MLV
TagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma5-GFP2
Plasmid#166777PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma5-GFP2 (GNG5 Human, Synthetic)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma3-GFP2
Plasmid#166775PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma3-GFP2 (GNG3 Human, Synthetic)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM-Venus
Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
TagsNanoLuc and mVenusExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-WT-V5
Plasmid#167468Purposeectopic expression of hDDB2 in mammalian cellsDepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-YFP
Plasmid#166897Purposemammalian expression of Sar1b tagged with YFPDepositorInsertSar1b
TagsYFPExpressionMammalianPromoterCMVAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 RBD (bio-His)
Plasmid#166857PurposeHuman expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.DepositorInsertSARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
Tags6xHis and Biotinylation siteExpressionMammalianPromoterCMVAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Tags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NLS
Plasmid#138217PurposeNuclear-targeted FRET-based PKA activity reporter.DepositorInsertAKAR4-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
TagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
eMagAF-EGFP-Mito
Plasmid#162243PurposeBlue-light dependent heterodimerization systemDepositorInserteMagAF-EGFP-OMP25
ExpressionMammalianPromoterCMVAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ609-dCas9-KR-puro
Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NUP188
Plasmid#159893PurposeExpresses NUP188 in mammalian cellsDepositorInsertNUP188 (NUP188 Human)
TagsEGFPExpressionMammalianMutationTo avoid NotI site, first three amino acids are d…PromoterCMVAvailable SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only