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lentiCRISPRv2_sgCtrl#2

Plasmid

#174149

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt1

Plasmid

#220244

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R27A, R31A, F165A

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK2

Plasmid

#172613

Purpose

Expresses HA-tagged CDK2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLCHKO_intergenic-intergenic_2

Plasmid

#155078

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mGSDME F2A

Plasmid

#154878

Purpose

Expresses mGSDME F2A in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Mutation

changes Phenylalanine 2 to Alanine

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

Plasmid

#208976

Purpose

Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

nSpCas9(H840)-BPNLS-P2A-GFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF846

Plasmid

#88355

Purpose

Donor Vector containing ZNF846 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF846 (ZNF846 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_NMNAT3_WT

Plasmid

#81885

Purpose

Gateway Donor vector containing NMNAT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NMNAT3 (NMNAT3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRecLV105 MEF2C

Plasmid

#107240

Purpose

A lentiviral vector for expression in mammalian cells encoding MEF2C insert

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRIM55_WT

Plasmid

#81829

Purpose

Gateway Donor vector containing TRIM55 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRIM55 (TRIM55 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP6C_WT

Plasmid

#81811

Purpose

Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP6C (PPP6C Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNK3_p.V488A

Plasmid

#81723

Purpose

Gateway Donor vector containing WNK3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNK3 (WNK3 Human)

Use

Gateway entry vector

Mutation

V488A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-T1977R

Plasmid

#69011

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-T1977R (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Threonine 1977 to Arginine

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
CMV-FLAG-TAOK2

Plasmid

#197862

Purpose

Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

Depositor

Jonathan Morris

Article

Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

Insert

TAOK2 (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_WT

Plasmid

#81787

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUt-mPF-GFP

Plasmid

#199222

Purpose

LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

rtTA-Advanced::P2A::EGFP

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-HP1a-mCherry

Plasmid

#185012

Purpose

Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

CBX5 (HP1a) (Cbx5 Mouse)

Expression

Bacterial

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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