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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDB in pMAX

Plasmid

#120403

Purpose

enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

Insert

EDB-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TMPO_WT

Plasmid

#81818

Purpose

Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TMPO (TMPO Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SAMHD1_WT

Plasmid

#81899

Purpose

Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SAMHD1 (SAMHD1 Human)

Use

Gateway entry vector

Mutation

G404E; A546V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

Plasmid

#204821

Purpose

Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

Depositor

Emma Rawlins

Article

He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

Insert

An upstream region including human SCGB3A2 promoter

Use

Lentiviral

Expression

Mammalian

Promoter

SCGB3A2 promoter

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNFRSF17_WT

Plasmid

#82263

Purpose

Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFRSF17 (TNFRSF17 Human)

Use

Gateway entry vector

Mutation

N81S; K86E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-METTL14-WT

Plasmid

#141112

Purpose

Overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPARG_WT

Plasmid

#82223

Purpose

Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPARG (PPARG Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-FCER1G-1

Plasmid

#109192

Purpose

Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Human full-length FCER1G, codon-optimized (FCER1G Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCA_WT

Plasmid

#82225

Purpose

Gateway Donor vector containing PRKCA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCA (PRKCA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBK1_WT

Plasmid

#82285

Purpose

Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBK1 (TBK1 Human)

Use

Gateway entry vector

Mutation

N388D; K570Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MLST8_WT

Plasmid

#82258

Purpose

Gateway Donor vector containing MLST8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MLST8 (MLST8 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_WT

Plasmid

#82221

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SOCS3_WT

Plasmid

#82245

Purpose

Gateway Donor vector containing SOCS3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SOCS3 (SOCS3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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