We narrowed to 3,198 results for: ER

1. pF(UG) hSyn PP-HaloTag-SYT7alpha

   Plasmid

   #179714

   Purpose

   Lentiviral expression of mouse Syt7 with N terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF(UG) hSyn PM-SYT7alpha deltaTMD

   Plasmid

   #179720

   Purpose

   Lentiviral expression of plasma membrane-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF NS34a-msGFP-'S-mito

   Plasmid

   #158765

   Purpose

   Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Flexible linkers between the protease and msGFP a…

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 SWS cone opsin

   Plasmid

   #109363

   Purpose

   Human SWS cone opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   SWS cone opsin (OPN1SW Human)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC-SmBiT

   Plasmid

   #207632

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-SmBiT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330-USP13

   Plasmid

   #61812

   Purpose

   to make USP13 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-optoB2-LYY-YFP

   Plasmid

   #75271

   Purpose

   G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   G-protein independent version of optoB2
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   point mutations at L72F,Y136G,Y224A

   Promoter

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDOC-K-glmS-mPlum

   Plasmid

   #158062

   Purpose

   Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   monomeric plum fluoresecent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   acpP

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-bsd-Flag-Trl1

    Plasmid

    #126609

    Purpose

    Expresses N-terminal Flag-tagged yeast Trl1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA ligase 1 (TRL1 Budding Yeast)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fast-GCaMP-EF05

    Plasmid

    #52558

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF05
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed from GCaMP3: D397N/G398A/N399D

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pB-puro-V5-Trpt1

    Plasmid

    #126607

    Purpose

    Expresses N-terminal V5-tagged mouse Trpt1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-USP44-HIS

    Plasmid

    #43894

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pILGFPU0 (R)

    Plasmid

    #83562

    Purpose

    Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBXNH3LCA_TM287/288

    Plasmid

    #132701

    Purpose

    expresses TM287/288 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    TM287/288 (TM0288, TM0287 Thermotoga maritima)
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cenp-B DBD INCENP mCherry

    Plasmid

    #45233

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD INCENP-Δcen mCherry
    

    Use

    Tags

    DNA binding domain of CENP-B and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET14_GFP5_Encap

    Plasmid

    #86408

    Purpose

    Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 5aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. sm-9xGCN4-Renilla-MS2

    Plasmid

    #178009

    Purpose

    Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Gomez-Puerta et al Elife. 2022 Jun 22;11. pii: 75580. doi:

    Insert

    sm-9xGCN4
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTorPE-Y-GECO2f

    Plasmid

    #105065

    Purpose

    A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2f
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLENTI CMV GFP-TREX1 V235fs BLAST

    Plasmid

    #164236

    Purpose

    lentiviral plasmid for GFP-TREX1 V235fs

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V235 frame shift mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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