We narrowed to 16,918 results for: Emb;

1. pAL950

   Plasmid

   #119748

   Purpose

   precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Internal NdeI site removed and mutations Alanine …

   Promoter

   T7

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS_AKAR5’

   Plasmid

   #114141

   Purpose

   Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5'
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAF210

   Plasmid

   #52406

   Purpose

   Expresses Flag-tagged mouse Arf5

   Depositor

   Gregory Pazour

   Article

   Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

   Insert

   Arf5 (Arf5 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJAF214

   Plasmid

   #52404

   Purpose

   Expresses Flag-tagged mouse Arf3

   Depositor

   Gregory Pazour

   Article

   Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

   Insert

   Arf3 (Arf3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Ydf NanoLuc

   Plasmid

   #225656

   Purpose

   Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NanoLuciferase
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   PydfJ115

   Available since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL NarX Myc

   Plasmid

   #225651

   Purpose

   Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarX (narX E. coli)
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

   Plasmid

   #207024

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   D384A Y387A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

   Plasmid

   #207023

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A I375A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

   Plasmid

   #207022

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Y387A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

    Plasmid

    #207021

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

    Plasmid

    #207020

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    I375A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

    Plasmid

    #207019

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. BS-TRP1-TetOff(7repeats)-GFP-Pho8

    Plasmid

    #207011

    Purpose

    Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ClhN-P1K-3VSV-Atg8-MET15

    Plasmid

    #207055

    Purpose

    Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    Promoter

    pATG8

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. P1K-3HA-Atg8∆R-MET15

    Plasmid

    #207034

    Purpose

    Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆R

    Promoter

    pATG8

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYea5.1_Notch1-L24_sfGFP

    Plasmid

    #207865

    Purpose

    Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
    

    Use

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYea5.1_Notch1_sfGFP

    Plasmid

    #207801

    Purpose

    Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein
    

    Use

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEVA424::tsi5

    Plasmid

    #192960

    Purpose

    Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tsi5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29a(+)::D1141A

    Plasmid

    #192963

    Purpose

    Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5_D1141A
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    D1141A

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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