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Plasmid#185010PurposeEncodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFPDepositorInsertTRIM28 sgRNA (Trim28 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-TUBA1B_sgRNA
Plasmid#183888PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorInsertTUBA1B sgRNA spacer (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC147-pCR8-dCas9VP64
Plasmid#48219PurposedCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP64ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-gfap-sgRNA
Plasmid#65566Purposein vitro trancription of sgRNA targeting the zebrafish gfap locusDepositorInsertgfap sgRNA (gfap Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11-MMEJ
Plasmid#207760PurposeMMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSNR52-sgTET
Plasmid#46923PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoterDepositorInsertsgRNA targeting endogenous TRE elements of pTET07 promoter
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE
Plasmid#192161PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-tmeGFP-IRES-nls-mRuby2
Plasmid#63060PurposeExpresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertnls-mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianMutationPromoterTet-OnAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-NFYC-IDR-mEGFP
Plasmid#185015PurposeBacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion ProteinDepositorInsertNFYC IDR (Nfyc Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA
Plasmid#192159PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
SL537
Plasmid#49945PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-4 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC148-pCR8-dCas9VP96
Plasmid#48220PurposedCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP96ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN (delta)N (aa1-117 deleted)
Plasmid#73382PurposeExpresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117DepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationDeleted N-terminal 1-117 aa from XPLN proteinPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa304-466 (PH)
Plasmid#73380PurposeExpresses amino acids 304-466 of XPLN (Arhgef3) proteinDepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses amino acids 304-466 of XPLN (arhgef3) p…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa1-125 (N125)
Plasmid#73378PurposeExpression of the N-terminal region of XPLN (amino acids 1–125, designated N125)DepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses N terminal amino acid 1-125 of XPLN (ar…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
SL534
Plasmid#49940PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-1 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 ATG wt
Plasmid#52034Purposeencodes wt HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationstarting ATG deletedPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
SL535
Plasmid#49941PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-2 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 ATG m7
Plasmid#52035Purposeencodes mutant HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationall seven let-7 sites in the 3′ UTR mutated (‘m7’)PromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only