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Plasmid#112031PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)DepositorInsert14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM
Plasmid#169982PurposeTet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAMPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG S297A
Plasmid#169949PurposeMammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297APromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG S297A
Plasmid#169992PurposeMammalian purification construct for CARD8 with an autoprocessing-deficient mutationDepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-siriusGFP-FLAGExpressionMammalianMutationS297APromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-5.3-GFP-HiBit
Plasmid#171014PurposeFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-5.3-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F405G
Plasmid#169956PurposeMammalian expression of CARD8-FLAG with a site III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationF405GPromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L370G
Plasmid#169970PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationL370GPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L368G
Plasmid#169969PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationL368GPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G
Plasmid#169960PurposeMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAG and UbExpressionMammalianMutationF370GPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX305-N-dTAG-CARD8-ZUC
Plasmid#169990PurposeMammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).DepositorInsertCARD8 (CARD8 Human)
UseTagsdTAGExpressionMammalianMutationPromoterhPGKAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM
Plasmid#169975PurposepDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAMPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM L368G
Plasmid#169978PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, L368GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM R394E
Plasmid#169980PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, R394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM S297A
Plasmid#169976PurposepDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, S297APromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R
Plasmid#169967PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationE274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E
Plasmid#169971PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationR394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G
Plasmid#169973PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationF405GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only