We narrowed to 2,403 results for: T2A

1. pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78307

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADflluw
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSC-ASCL1-IRES-GFP-T2A-Sox11

   Plasmid

   #182233

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   ASCL1-IRES-GFP-T2A-Sox11
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

   Plasmid

   #193915

   Purpose

   Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-MND-hCard11 R72L-T2A-GFP

   Plasmid

   #163784

   Purpose

   Expression of R72L CARD domain variant

   Depositor

   Richard James

   Article

   Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

   Insert

   CARD11 (CARD11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R72L

   Promoter

   MND

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-mouse SmyD1-T2A-copGFP

   Plasmid

   #101157

   Purpose

   Lentiviral expression of SmyD1 and copGFP

   Depositor

   Martine Roussel

   Article

   Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/o

   Insert

   SmyD1 (Smyd1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-FNLS-T2A-EGFP-ires-puro

   Plasmid

   #121169

   Purpose

   CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   FNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn1 bPAC cMyc T2A tDimer

   Plasmid

   #85397

   Purpose

   humanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent protein

   Depositor

   Thomas Oertner

   Inserts

   humanized photoactivated adenylyl cyclase
   red fluorescent protein
   

   Use

   AAV

   Tags

   cMyc Tag

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin-1 and ribosomal skip sequence T2A

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

   Plasmid

   #79913

   Purpose

   piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

   Depositor

   Joshua Sanes

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Zeocin-t2A-sHRPbNRX3beta
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

   Plasmid

   #129048

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA8 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129036

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid

    #129053

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129029

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

    Plasmid

    #79914

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE

    Plasmid

    #113853

    Purpose

    Superfolder GFP and tdTomato linked by T2A (V linker)

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry

    Plasmid

    #89057

    Purpose

    PV4 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    GFP-T2A-mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti poly-ubiquitin

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

    Plasmid

    #187079

    Purpose

    Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dHaloTag7 = HaloTag7-D106A

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1α-DIO-cOpn5-T2A-EGFP

    Plasmid

    #237858

    Purpose

    The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

    Depositor

    Minmin Luo

    Article

    Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

    Insert

    Opn5 (OPN5 Chicken)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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