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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hU6

Plasmid

#102843

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

As pcr template

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

Plasmid

#248211

Purpose

bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEGFP-K49R-STAT3

Plasmid

#71447

Purpose

Retroviral expression of K49R-STAT3. Please note that this plasmid contains K49R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Publication

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

K49R

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCK123 pDESTattB_exorh:mCerulean

Plasmid

#213512

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Publication

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTattB_NSnoFbox-vhhGFP4

Plasmid

#35578

Depositor

Markus Affolter

Publication

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSnoFbox-vhhGFP4 (slmb Fly)

Expression

Insect

Mutation

F box domain of slmb deleted.

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Publication

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Expression

Mammalian

Promoter

TRE3G

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector

Plasmid

#99292

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLEGFP-K140R-STAT3

Plasmid

#71448

Purpose

Retroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Publication

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

K140R

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
STARD5A

Plasmid

#42392

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

STARD5A (STARD5 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
qTAG-C-mStayGold-Blast

Plasmid

#227254

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro

Plasmid

#227253

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Distal Promoter-FLuc2P

Plasmid

#154260

Purpose

Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

Depositor

Antonio Amelio

Publication

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Distal Promoter (PPARGC1A Human)

Expression

Mammalian

Promoter

Human PGC-1alpha Distal Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Quas:GFPpA;Betacrystallin:CFP

Plasmid

#81181

Purpose

Replaces #61376. QUAS driving GFP with beta crystalline CFP eye marker

Depositor

Marnie Halpern

Insert

QUAS:GFP;beta crys:CFP

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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qTAG-N-Puro-mStayGold

Plasmid

#227250

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH

Plasmid

#184463

Purpose

pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.

Depositor

Rhonda Kineman

Publication

Lau-Corona et al Endocrinology. 2022 Apr 9. pii: 6565757.

Insert

N-Flag-mSTAT5bCA (Stat5b Mouse)

Use

AAV

Tags

FLAG Tag

Expression

Mammalian

Mutation

Point mutation at base 1963 (A to C), which chang…

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGSTag Flag Jnk2a2

Plasmid

#15744

Depositor

Roger Davis

Publication

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Expression

Bacterial

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223_STAT6_p.N417Y

Plasmid

#81591

Purpose

Gateway Donor vector containing STAT6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT6 (STAT6 Human)

Use

Gateway Cloning

Mutation

N417Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT1_p.Y701F

Plasmid

#82183

Purpose

Gateway Donor vector containing STAT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT1 (STAT1 Human)

Use

Gateway Cloning

Mutation

Y701F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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