We narrowed to 3,175 results for: tef
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Plasmid#89577PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locusDepositorInsertAde2 gene drive
UseSynthetic BiologyExpressionYeastAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1510
Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
ExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63Available sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVW300
Plasmid#139495PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpTEF PP7∆FG-GFPenvy tCYC1
ExpressionYeastMutation∆FGPromoterpTEFAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRExpressionBacterial and YeastAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP (pJJF001)
Plasmid#173457PurposeC-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)Available sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKT-SCFP3A-HIS
Plasmid#64686PurposepKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.DepositorInsertSCFP3A
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKT0101
Plasmid#8721DepositorPublicationInsertyECFP
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163AAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0212
Plasmid#8725DepositorPublicationInsertyECFP A206R
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…Available sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYES2-gRNA-hyg-MCS
Plasmid#107734PurposeE. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.DepositorInsertX-3 site gRNA expression cassettes
UseCRISPRExpressionYeastPromoterSNR52pAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEL15
Plasmid#107921Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH3FS-GAL1
Plasmid#85782PurposeN-ICE plasmid pH3FS-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-01
Plasmid#36217DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertyEGFP
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-kanMXAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorPublicationInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only