We narrowed to 6,574 results for: val

1. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-His6-OAZ-GSY-SPM-Ctag

   Plasmid

   #163613

   Purpose

   Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

   Depositor

   Mark Howarth

   Article

   Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

   Insert

   OAZ-GSY-SPM
   

   Use

   Tags

   C-tag and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 416-Met25 Kar2 printor HDEL

   Plasmid

   #32129

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   Printor (KLHL14 Human)
   

   Use

   Tags

   HDEL and Kar2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

4. MDH1-PGK-FLAG-TAT-86-E

   Plasmid

   #35978

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "E" HIV clade

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX-FLAG-RHEB-IRES-Puro

   Plasmid

   #120576

   Purpose

   Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. K347CLM G234A/43/347

   Plasmid

   #24454

   Depositor

   Ron Vale

   Article

   Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

   Promoter

   Available since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-Hook3 aa 1-160

   Plasmid

   #74607

   Purpose

   Expresses Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-GFP-Patronin CC 638–1457

   Plasmid

   #59051

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pENTR-GFP-Patronin CC 776–1457

   Plasmid

   #59052

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

10. MDH1-PGK-FLAG-TAT-SIVmac251

    Plasmid

    #35979

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    transactivating protein
    

    Use

    RNAi and Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    SIV strain mac251

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS426-TUB1-internal6xHis (1-440)

    Plasmid

    #60391

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. K349CLM-6His 215/347

    Plasmid

    #24451

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

13. A337C/Y344C

    Plasmid

    #24434

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

14. K222C/E334C

    Plasmid

    #24435

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pRS424-TUB2 - human TUBB2a Cterminal tail

    Plasmid

    #60396

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. K420CLM RP

    Plasmid

    #24443

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. CLM RP KGF Mut 1

    Plasmid

    #24166

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Promoter

    Available since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET-Impact-T4gp61(I342G)

    Plasmid

    #228208

    Purpose

    Expression in BL21(DE3) of T4 primase I342G

    Depositor

    Stephen Benkovic

    Article

    Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

    Insert

    61 (61 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated isoleucine 342 to glycine (I342G), improv…

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTSAR3.6t

    Plasmid

    #210258

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mScarlet
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTSAR3.7t

    Plasmid

    #210259

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    e2Crimson
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge