167,948 results

1. pAAV-Ef1a-Con/Foff 2.0-mCherry

   Plasmid

   #137133

   Purpose

   Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

   Plasmid

   #101297

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-906 of alpha-Catenin

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-HS-Tn5

   Plasmid

   #127916

   Purpose

   Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Hyperstable Tn5
   

   Tags

   E coli elongation factor Ts and Mxe intein - Chit…

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038)

   Plasmid

   #191720

   Purpose

   AI plasmid ALIAS: AiP13038. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   none

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIC162

   Plasmid

   #63070

   Purpose

   Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

   Plasmid

   #139994

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lys-eMagA-EGFP

   Plasmid

   #162246

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   p18-eMagA-EGFP
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSSRB069_pAC8-hsDDB1dB

   Plasmid

   #218790

   Purpose

   Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Deleted amino acids 396-705 and replaced with GNG…

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Caspase-Reporter

    Plasmid

    #243673

    Purpose

    Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

    Depositor

    Matthias Wirth

    Article

    Selcen et al Cell Death Discov, 11, 368, 06 August 20

    Insert

    Split-GFP with DEVD sequence and T2A mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK7-HA-S6K1-F5A-E389-R3A

    Plasmid

    #8991

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_BiSSTe10_C1V1_eYFP

    Plasmid

    #213816

    Purpose

    AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_C1V1_eYFP

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    C1V1-eYFP
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

    Plasmid

    #203539

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-mScarlet-2A-mScarlet
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

    Plasmid

    #223174

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-PRKAR1A

    Plasmid

    #23741

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRKAR1A (PRKAR1A Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mem-TurboID

    Plasmid

    #149409

    Purpose

    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Tags

    HA and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 Flag TSC2 (S939A/T1462A)

    Plasmid

    #14131

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A/T1462A (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A/T1462A

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pGT-Ah7

    Plasmid

    #122574

    Purpose

    MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    catP/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Expression

    Insect

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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