We narrowed to 13,693 results for: crispr cas9

1. pAT-9218

   Plasmid

   #124225

   Purpose

   Bacterial SpCas9 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   TetR/TetA

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_DNMT3b

   Plasmid

   #41823

   Purpose

   Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_DNMT3b (DNMT3B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pGC(2.1)-U6.3

   Plasmid

   #170516

   Purpose

   PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   gRNAcore(2.1)-pU6.3
   

   Use

   Pcr template vector for amplifying grnacore(2.1)-…

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSAC-Crys

   Plasmid

   #164967

   Purpose

   Expresses sgRNA in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   hU6, hSyn

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. VQR variant

   Plasmid

   #138560

   Purpose

   Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CSAC-Bmal1

   Plasmid

   #168296

   Purpose

   Expresses sgRNA in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgBmal1-1-hU6-sgBmal1-3
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   U6, hSyn

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQD1CB0202

   Plasmid

   #185626

   Purpose

   MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE Cacnb2-GFP KI

   Plasmid

   #139661

   Purpose

   Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO1-puro-U6-sgRNA-LRTM2

    Plasmid

    #69237

    Purpose

    U6 driven SpCas9 sgRNA expression for LRTM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    LRTM2 sgRNA (LRTM2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX552 EF1a DIO Chronos GFP_Vgat gRNA

    Plasmid

    #215277

    Purpose

    Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Vgat gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

    Plasmid

    #215278

    Purpose

    Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Vgat control gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

    Plasmid

    #215281

    Purpose

    Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gly-tRNA-gRNA scaffold for library 2

    Plasmid

    #192365

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pro-tRNA-gRNA scaffold for library 2

    Plasmid

    #192366

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gly-tRNA-gRNA scaffold for library 1

    Plasmid

    #192368

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pro-tRNA-gRNA scaffold for library 1

    Plasmid

    #192369

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAT-9251

    Plasmid

    #124226

    Purpose

    Bacterial SpCas9-HF1 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-ABE-P48R

    Plasmid

    #161816

    Purpose

    Expresses ABE-P48R in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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