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MTK234_008

Plasmid

#123902

Purpose

Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GFP dropout for multiTU destination vectors

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp2-GFPuv

Plasmid

#139083

Purpose

Low phosphate inducible promoter yibDp2 for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yibDp2-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A3701

Plasmid

#91052

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-dAtCas9-VPR

Use

CRISPR

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C3003

Plasmid

#91095

Purpose

Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

CmYLCV

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B(T116C)

Plasmid

#157808

Purpose

Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B(T116C)

Expression

Bacterial

Mutation

T116C

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b LFn-Fla

Plasmid

#84871

Purpose

Bacterial expression of LFn-Fla fusion

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pMOD_A0502

Plasmid

#91012

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_VDR_Hormone-recep

Plasmid

#109962

Purpose

Protein expression and purification of VDR_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VDR_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Orai1-myc E106Q MO70

Plasmid

#22754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E106Q (ORAI1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

E106Q

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pMOD_C3006

Plasmid

#91096

Purpose

Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSynCAM 4

Plasmid

#115499

Purpose

expression of mouse full-length SynCAM 4

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm4 (Cadm4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-subtilase A

Plasmid

#35948

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

Subtilase A (subA E. Coli)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-NHERF1-PDZ1-2

Plasmid

#28294

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-298 (deletion of the EB domain aa 2…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPF45

Plasmid

#110044

Purpose

Protein expression and purification of SPF45

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPF45 (RBM17 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMB-TMEr

Plasmid

#61208

Purpose

Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry-BCP

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

Plasmid

#193852

Purpose

Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP-CAAX

Plasmid

#75176

Purpose

Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUBC_hsPUM1-iRFP670

Plasmid

#107609

Purpose

Transient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoter

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

hsPUM1-iRFP670

Use

Synthetic Biology

Tags

iRFP670

Expression

Mammalian

Promoter

human UBC

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-Dendra

Plasmid

#140559

Purpose

Red to green photoconvertible chromosome marker

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

H2B

Tags

Dendra

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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