167,948 results

1. pMXs-IP-mVenus-TOSI

   Plasmid

   #172491

   Purpose

   mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Pdcd4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   MLV LTR

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CARPID dCasRx-BASU

   Plasmid

   #153303

   Purpose

   express CARPID dCasRx-BASU fusion protein in mammalian cells

   Depositor

   Jian Yan, Liang Zhang

   Article

   Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

   Insert

   dCasRx-BASU
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A in RfxCas13d

   Promoter

   EF-1a

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TUBB5-Halo

   Plasmid

   #64691

   Purpose

   Expresses beta-tubulin labeled with Halo-tag

   Depositor

   Yasushi Okada

   Article

   Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

   Insert

   TUBB
   

   Tags

   Halo-tag

   Expression

   Mammalian

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

   Plasmid

   #228469

   Purpose

   Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

   Depositor

   Mary Dunlop

   Article

   Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

   Inserts

   w4-loxP-TT-loxP-mCherry
   Ho1
   PcyA
   

   Expression

   Bacterial

   Promoter

   J23106, J23108, and T7A1

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn1-tTA

   Plasmid

   #99119

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   hSyn1

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA Raptor

   Plasmid

   #8513

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Tags

   ha

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc-GFPC1-Sec61beta

   Plasmid

   #15108

   Depositor

   Tom Rapoport

   Insert

   Sec61beta (SEC61B Human)
   

   Tags

   Ac GFP

   Expression

   Mammalian

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 TEV LIC cloning vector (1B)

   Plasmid

   #29653

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

    Plasmid

    #120563

    Purpose

    Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN.AAV.CMVs.TurboRFP.WPRE.RBG

    Plasmid

    #105548

    Purpose

    AAV expression of TurboRFP from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV8

    Insert

    TurboRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF4Ag NanoLuc (ATG-42)

    Plasmid

    #137777

    Purpose

    Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

    Depositor

    Lance Encell

    Article

    Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

    Insert

    NanoLuc (Nluc)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NanoLuc contains 16 amino acid changes compared t…

    Promoter

    T7 and CMV

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-APOBEC1-YTH

    Plasmid

    #131636

    Purpose

    Expresses APOBEC1 fused to the YTH domain

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1 fused to YTH domain (YTHDF2 Rat, Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. iRFP-H2A

    Plasmid

    #158691

    Purpose

    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    iRFP670
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-ER-mTagBFP2

    Plasmid

    #216127

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMHE-miRFP-MAP4-MTBD

    Plasmid

    #171487

    Purpose

    T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    miRFP

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. hiActTS-GR

    Plasmid

    #79775

    Purpose

    high FRET control for ActTS-GR

    Depositor

    Tetsuya Kitaguchi

    Article

    Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

    Insert

    hiActTS-GR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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