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pRPR1(1xTetO)_gRNA_handle_RPR1t

Plasmid

#62966

Purpose

aTc inducible gRNA expression vector

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Expression

Yeast

Promoter

RPR1

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pIT5_KH_VanR

Plasmid

#173779

Purpose

Bacterial genomic integration vector, VanR repressor

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Tags

paGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pREMI-Z

Plasmid

#59527

Purpose

Zeocin-resistance plasmid for insertional mutagenesis.

Depositor

Benjamin Glick

Article

Mukaiyama et al Genes Cells. 2002 Jan;7(1):75-90.

Type

Empty backbone

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pS3aG

Plasmid

#31171

Purpose

A Drosophila GFP reporter transgene vector for site-specific integration

Depositor

Thomas Williams

Article

Williams et al Cell. 2008 Aug 22;134(4):610-23. doi: 10

Type

Empty backbone

Expression

Insect

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only
pEB587

Plasmid

#22065

Depositor

Emmanuelle Bouveret

Article

Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

Type

Empty backbone

Tags

TAP

Expression

Bacterial

Available Since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pG20_MCS_blp

Plasmid

#159710

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO43.3.-6_human ProCas9Flavi-6

Plasmid

#121630

Purpose

U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMOLUC

Plasmid

#12514

Depositor

Tong-Chuan He

Article

Feng et al Biotechniques. 2002 May . 32(5):992, 994

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

Plasmid

#44093

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pJMP2782

Plasmid

#160668

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCARSF85

Plasmid

#64694

Purpose

H6-TF-Smt3-GB1-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, NpuDnaE-N102, Smt3, and TF

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_Blp

Plasmid

#159702

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mCherry

Expression

Plant

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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21 pCOLI_A_N-GST_C_STREP

Plasmid

#160258

Purpose

N-term GST 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII and GST

Expression

Bacterial

Promoter

T7

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGMCZq17-OXOX

Plasmid

#31289

Depositor

Dirk Schnappinger

Article

Venugopal et al Cell Host Microbe. 2011 Jan 20. 9(1):21-

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Article

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-miR-20-Sensor

Plasmid

#21168

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Type

Empty backbone

Expression

Mammalian

Available Since

June 10, 2009

Availability

Academic Institutions and Nonprofits only
pTALYM9

Plasmid

#47876

Purpose

Destination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assembly

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Type

Empty backbone

Use

TALEN

Tags

Ty1tag

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAd-Track Control siRNA

Plasmid

#8437

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Type

Empty backbone

Use

Adenoviral and RNAi

Expression

Mammalian

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pQM

Plasmid

#48097

Purpose

Cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and myc

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 8, 2013

Availability

Academic Institutions and Nonprofits only

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