We narrowed to 59,251 results for: {{{s}}}

1. moPrP(3F4) D177N

   Plasmid

   #1319

   Depositor

   Susan Lindquist

   Article

   Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

   Insert

   PrP D177N (Prnp Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D177N point mutation

   Promoter

   Available since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-LGC-53

   Plasmid

   #86399

   Purpose

   Mammalian expression of codon-optimized dopamine receptor LGC-53

   Depositor

   Steven Mennerick

   Article

   Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

   Insert

   LGC-53 (lgc-53 Nematode, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for mammalian expression; Signal …

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15

   Plasmid

   #153523

   Purpose

   Expression of the anti SARS-CoV-2_RBD sybody Sb#15

   Depositor

   Markus Seeger

   Article

   Walter et al bioRxiv 2020.04.16.045419

   Insert

   Sb#15
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. asPink chromoprotein promoter-less

   Plasmid

   #118005

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, asPink
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+ Zf cdh1-deltaCyot-GFP

   Plasmid

   #185412

   Purpose

   GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   cdh1 (cdh1 Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain deleted

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV P2Gm p53

   Plasmid

   #19751

   Depositor

   Richard Hynes

   Article

   Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   oligo targeting p53 (Trp53 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)-FluoSTEP-AktAR

   Plasmid

   #181856

   Purpose

   Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AktAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Use

   Tags

   Strep II tag on Cas1

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pM124: pAAV-EFS-CasRx-VEGFA presgRNA

    Plasmid

    #166872

    Purpose

    AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-VEGFA presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY118A

    Plasmid

    #182958

    Purpose

    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Inserts

    Cas9
    Gam, Beta, Exo
    I-scel
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFX-mito-at pHluorin

    Plasmid

    #174183

    Purpose

    Express EYFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    at pHluorin with mitchondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGTag-P2A-QFGal4-SV40pA

    Plasmid

    #155127

    Purpose

    Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    P2A-QFGal4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-AC3CtermGenomic-mCherry

    Plasmid

    #105250

    Purpose

    mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eNme2-C TadCBEa

    Plasmid

    #193840

    Purpose

    Express TadCBEa (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS303 Gal TDP-43 YFP

    Plasmid

    #27468

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    D23A mutation in TDP-43 sequence

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pHAGE-FKBP-GFP-OPTN (2-119)

    Plasmid

    #208867

    Purpose

    Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    OPTN (OPTN Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    aa 2-119 only

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GW1-mito-mCherry-roGFP2

    Plasmid

    #107220

    Purpose

    Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    mCherry-roGFP2
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Beclin1 CC

    Plasmid

    #24403

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains the CC domain (aa 151-242)

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pmRFP-SUMO1 GG

    Plasmid

    #13990

    Depositor

    Jonathan Chernoff

    Article

    Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

    Insert

    SUMO1 GG (Sumo1 Mouse)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    C-terminal truncation. Cannot ligate to substrate…

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only