We narrowed to 15,730 results for: NOL

1. PB-TRE-ETV2

   Plasmid

   #225051

   Purpose

   PiggyBac vector encoding doxycycline-inducible codon-optimized human ETV2

   Depositor

   Sean Palecek, Eric Shusta

   Article

   Ding et al Biotechnol Bioeng. 2025 Jul;122(7):1914-

   Insert

   ETS Variant Transcription Factor 2 (ETV2 Human)
   

   Expression

   Mammalian

   Mutation

   Codon Optimized

   Promoter

   TRE3G

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL-SFFV_V5-CHIC2-C6S_Tomato

   Plasmid

   #238128

   Purpose

   Expresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

   Insert

   V5-tagged CHIC2 with C6S mutation
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed cysteines from amino acids 102-109 to ser…

   Promoter

   SFFV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-SFFV_STUB1-UBOXdel-V5_Tomato

   Plasmid

   #238129

   Purpose

   Expresses V5-tagged STUB1 (with UBOX deletion) with Tomato selectable marker.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

   Insert

   V5-tagged-STUB1
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deleted UBOX domain (amino acids 224-294)

   Promoter

   SFFV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HaloAKAR2.0-T2A-EGFP

   Plasmid

   #216488

   Purpose

   Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.0
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

   Plasmid

   #235608

   Purpose

   Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
   

   Use

   CRISPR

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBX_RPL29_smGFP_V5

   Plasmid

   #236091

   Purpose

   Constitutively encodes RPL29-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting RPL29 in the 60S subunit.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   RPL29-smGFP-V5 (RPL29 Human, Synthetic)
   

   Expression

   Mammalian

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGEN_NES_V5_eMagAF_C74_MAVS

   Plasmid

   #235638

   Purpose

   Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MAVS residues 500-540 for targeting to outer mitochondrial membrane

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   ALIBi C-terminal fragment
   

   Tags

   MAVS and NES-V5-eMagAF

   Expression

   Mammalian

   Mutation

   residues 74-321

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

   Plasmid

   #231360

   Purpose

   Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   BC(p1-10)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

   Plasmid

   #231361

   Purpose

   Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   BC(p1-10)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.minBG-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231362

    Purpose

    minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231363

    Purpose

    Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231368

    Purpose

    Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

    Plasmid

    #231351

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

    Plasmid

    #231354

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231345

    Purpose

    Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA528 huPQBP1

    Plasmid

    #217449

    Purpose

    Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    PQBP1 (PQBP1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. HIV-1-GFP ΔEnv CA E45A

    Plasmid

    #217438

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA E45A

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. HIV-1-GFP ΔEnv CA M66I

    Plasmid

    #217440

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA M66I

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. SG3ΔENV CA Q67H/N74D

    Plasmid

    #217442

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA Q67H,N74D

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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