We narrowed to 1,124 results for: Amot;

1. pNM1325

   Plasmid

   #197144

   Purpose

   CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   SpCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Promoter

   CAG

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-CS-196

   Plasmid

   #197138

   Purpose

   CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CS-117

   Plasmid

   #197134

   Purpose

   CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   EGFP(M1V)

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-CS-060

   Plasmid

   #197133

   Purpose

   CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   MCP(N55K)

   Promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-CS-193

   Plasmid

   #197137

   Purpose

   CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mCherry(M1V/M9A)

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-CS-119

   Plasmid

   #197135

   Purpose

   CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   EGFP(M1V)

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSpCas9-EF-5Flnc

   Plasmid

   #174870

   Purpose

   CRISPR vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Flnc (Flnc Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.2-coHPDL H163A H258A 3xFLAG-V5

   Plasmid

   #174131

   Purpose

   Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized, H163A, H258A

   Promoter

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6.2-coHPDL H258A 3xFlag-V5

   Plasmid

   #174130

   Purpose

   Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized, H258A

   Promoter

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK262 (RAD21-mAC Neo)

    Plasmid

    #140538

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid

    #207482

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    GFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Potential silent point mutation in GFP sequence

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid

    #212022

    Purpose

    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GianCreg (GOLGB1 Human)
    

    Use

    Tags

    td5StayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CTCF-mAC donor (Hygro)

    Plasmid

    #140646

    Purpose

    CTCF tagging with mAID-clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRXIP-GFP-TRPML1(PHD)

    Plasmid

    #221752

    Purpose

    Stable expression of GFP-TRPML1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TRPML1(PHD) (amino acids 1–69)

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ALCAM-p2A-E2Crimson

    Plasmid

    #83939

    Purpose

    Lentiviral vector expressing ALCAM-p2A-E2Crimson

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    ALCAM (ALCAM Human)
    

    Use

    Lentiviral

    Tags

    p2A-E2Crimson

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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