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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 961 - 980 of 2493 results

  5. pMOD_A0801

    Plasmid
    #91022
    Purpose
    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-AtCas9_dead (D10A + H840A)
    Use
    CRISPR
    Mutation
    D10A, H840A
    Promoter
    35S
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91022-plasmid-map-sequence-id-181775

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  6. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Publication
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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  7. VP64-dCas9

    Plasmid
    #177171
    Purpose
    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VP64-dCas9(D10A, H840A)
    Use
    CRISPR
    Tags
    NLS and NLS-3xFLag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177171-plasmid-map-sequence-id-348230

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  9. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161817-plasmid-map-sequence-id-320651

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  11. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

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  13. pKb-POC1-CBE

    Plasmid
    #255131
    Purpose
    Plant expression of A3A(Y130F)-OpenCRISPR1 D10A-UGI for cytosine base editing.
    Depositor
    Kutubuddin Molla
    Publication
    Das et al New Phytol. 2026 May 21. doi: 10.1111/np
    Insert
    A3A(Y130F) fused at 5' of the Rice codon optimized pOC1 D10A and UGI at the 3' end
    Tags
    NA
    Expression
    Plant
    Promoter
    Maize ubiquitin promoter for A3A(Y130F)-OpenCRISP…
    Available since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    255131-plasmid-map-sequence-id-516879

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  14. pSCBE3

    Plasmid
    #133966
    Purpose
    Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Zhong et al bioRxiv 630137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133966-plasmid-map-sequence-id-266621

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  18. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP96
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

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Showing: 961 - 980 of 2493 results