Addgene

We narrowed to 21,853 results for: ESC;

Showing: 961 - 980 of 21853 results

Results per page:

Sort results by:

pLenti_CMV:Flag-dKeNLuc

Plasmid

#208856

Purpose

Expresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

dKeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-CeNLuc

Plasmid

#208838

Purpose

Expresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-CeNL

Plasmid

#208839

Purpose

Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-GeNL

Plasmid

#208841

Purpose

Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-6-AmCyan-P2A-NptII-9

Plasmid

#197742

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AmCyan-P2A-NptII bicistronic CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF-p15A-T7- YFP

Plasmid

#186568

Purpose

p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-RCC

Plasmid

#190635

Purpose

Mammalian expression of genetically encoded calcium indicator RCC

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

RCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_H2B-STf

Plasmid

#181998

Purpose

CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

H2B-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-sg-1

Plasmid

#190607

Purpose

Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-sgNCF1

Plasmid

#190193

Purpose

Expresses a gRNA for base editing the Kozak sequence of the human NCF1 gene

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-SCcpV

Plasmid

#190636

Purpose

Mammalian expression of genetically encoded calcium indicator SCcpV

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCcpV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-Clover4-LUMABS-HIV

Plasmid

#183485

Purpose

To express the Clover4-LUMABS-HIV sensor for Acquired Immunodeficiency Virus antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-HIV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-Clover4-LUMABS-S1 RBD

Plasmid

#183491

Purpose

To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-S1 RBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-Clover4-LUMABS-EBNA1

Plasmid

#183488

Purpose

To express the Clover4-LUMABS-EBNA1 sensor for Epstein-barr Virus antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-EBNA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-Clover4-LUMABS-VCA

Plasmid

#183489

Purpose

To express the Clover4-LUMABS-VCA sensor for Epstein-barr Virus antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-VCA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCML 313

Plasmid

#98586

Purpose

pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETa-Amrose v2

Plasmid

#79538

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 2

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v2

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB786

Plasmid

#60151

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB682

Plasmid

#60143

Purpose

Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Use

Tags

PEST degron

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean-Keratin-17

Plasmid

#55379

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Keratin (KRT18 Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only