We narrowed to 2,722 results for: LRE

Showing: 961 - 980 of 2722 results

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
P[acman]-Alpha2

Plasmid

#165859

Purpose

Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-mTSP33

Plasmid

#236541

Purpose

For expression of Tspan33 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN33 (Tspan33 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp6

Plasmid

#163590

Purpose

Expresses BMP-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp6 (Bmp6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-TCAB1 HRD

Plasmid

#207535

Purpose

Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM55297

Plasmid

#153213

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

Plasmid

#62957

Purpose

Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4 in three phases

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Donor-PIGA

Plasmid

#153530

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 1,991-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex4

Plasmid

#85534

Purpose

Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-1

Plasmid

#74960

Purpose

Cas9 + sgGFP-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgMIB1

Plasmid

#140239

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

sgRNA targeting human MIB1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf5

Plasmid

#163596

Purpose

Expresses GDF-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf5 (Gdf5 Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165887

Purpose

G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

Plasmid

#124861

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

Plasmid

#114212

Purpose

Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pF208wt-luc

Plasmid

#122143

Purpose

For luciferase expression

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Fam208a promoter (Tasor Mouse)

Use

Luciferase

Promoter

Fam208a

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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RT3GEN

Plasmid

#111165

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Promoter

T3G

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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