Addgene

We narrowed to 7,585 results for: Lif;

Showing: 961 - 980 of 7585 results

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P195

Plasmid

#139716

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A''_p35S:AsNpga:tAtug7

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C)

Plasmid

#79047

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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P364

Plasmid

#139721

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A'_pSlH4:NnCPH:tSlH4

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

SlH4

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn_NES-His-rsCaMPARI-mRuby3

Plasmid

#120805

Purpose

AAV plasmid for expressing rsCaMPARI in neurons, nucleus excluded

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

AAV

Tags

NES-His and mRuby3

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

Plasmid

#140153

Purpose

Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDN3.1(+)-CaNARi

Plasmid

#64848

Purpose

FRET-based calcineurin (CaN) activation biosensor.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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U6-Nme2_tracr

Plasmid

#192146

Purpose

Expresses Nme2-sgRNA cloned with BspQI

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nme2 sgRNA scaffold

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDRFLIP34

Plasmid

#65512

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV hSynapsin flexed SV-tag

Plasmid

#163686

Purpose

express SV-Tag construct in a Cre-dependent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synaptophysin 9X HA

Use

Tags

9X HA

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDC20

Plasmid

#131366

Purpose

expression of MEG-3 (aa1-862) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa1-862) (meg-3 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27G2)_FLAG

Plasmid

#199616

Purpose

Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27G2

Use

Tags

8xHis and FLAG

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pKL13-MgtC bb118

Plasmid

#158982

Purpose

Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mgtC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDRFLIP37

Plasmid

#65515

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-Cit-attR1 and attR2-Cer-cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP36

Plasmid

#65514

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-AFPt9-attR1 and attR2-Cer-cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Dcp2 FL

Plasmid

#163574

Purpose

express wild type Dcp2 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

DCP2 promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDRFLIP43

Plasmid

#65519

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-edAFPt9-attR1 and attR2-edCer-cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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