We narrowed to 3,301 results for: actin

1. GFP-Dynamin 2 K44A

   Plasmid

   #22301

   Depositor

   Pietro De Camilli

   Article

   Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

   Insert

   Dynamin 2 K44A (Dnm2 Rat)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   K44A

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-HA-ARHGAP29

   Plasmid

   #104154

   Purpose

   mammalian expression of HA tagged ARHGAP29

   Depositor

   Marius Sudol

   Article

   Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

   Insert

   ARHGAP29 (ARHGAP29 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b_RanT24N

   Plasmid

   #186271

   Purpose

   Expression vector of His-RanT24N in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Tags

   His tagged and His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed T24 into N

   Promoter

   T7 promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCD4-TagRFP-T (RHPMP151)

   Plasmid

   #119238

   Purpose

   Expresses CD4 fused to TagRFP-T

   Depositor

   Ricardo Henriques

   Article

   Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

   Insert

   CD4 (CD4 Human)
   

   Tags

   tagRFP-T

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174645

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
   

   Tags

   SSPB(micro)-Venus and iLID-mCherry

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti Dest PuroR R1-R2

   Plasmid

   #84575

   Purpose

   Gateway destination vector expressing Puromycin resistance under a PGK promoter

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB80-hsKIF5B(1-560)-L-GFP

   Plasmid

   #193716

   Purpose

   Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560);

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. phage - ubc - nls - 2xmcp - egfp - BirA*

   Plasmid

   #131132

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Article

   Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

   Insert

   NLS-HA-2xMCP-EGFP-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLXSN-H-Ras_N17

   Plasmid

   #39537

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS N17 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N17

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

10. AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173862

    Purpose

    Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. cpGFP-Adhiron_flex

    Plasmid

    #165044

    Purpose

    Flexible linker control for POLArISact expression in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    cpGFP-Adhiron (connected with a flexible linker)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-ACTB_sgRNA

    Plasmid

    #183885

    Purpose

    pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. S142A drebrin-YFP

    Plasmid

    #58335

    Purpose

    mammalian expression of human drebrin-YFP phospho-dead mutant

    Depositor

    Phillip Gordon-Weeks

    Article

    Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

    Insert

    drebrin (DBN1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    S142A, phospho-dead mutant

    Promoter

    CMV

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. S142D drebrin-YFP

    Plasmid

    #58336

    Purpose

    mammalian expression of human drebrin-YFP phospho-mimetic mutant

    Depositor

    Phillip Gordon-Weeks

    Article

    Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

    Insert

    drebrin (DBN1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    S142D, phospho-mimetic mutant

    Promoter

    CMV

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

15. EHD2-T72A-mEGFP

    Plasmid

    #45934

    Purpose

    dominant-negative EHD2 mutant

    Depositor

    Ari Helenius

    Article

    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

    Insert

    Eps-15 homology domain-containing protein2 (EHD2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    T 72 changed to A (T72A)

    Promoter

    CMV

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. M3-PSCFP2

    Plasmid

    #114185

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Tags

    3xHA and PS-CFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EHD2-T72A-mCherry

    Plasmid

    #45935

    Purpose

    dominant-negative EHD2 mutant

    Depositor

    Ari Helenius

    Article

    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

    Insert

    Eps-15 homology domain-containing protein2 (EHD2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T 72 changed to A (T72A)

    Promoter

    CMV

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV5-Flag-EPLIN alpha delta LIM

    Plasmid

    #40933

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted- amino acids 221-295

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLXSN-H-Ras_V12

    Plasmid

    #39516

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV5-Flag-EPLIN alpha delta C-term

    Plasmid

    #40930

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 296-600

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only