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pQCi-hTRAC-sgRNA

Plasmid

#154093

Purpose

pQCi backbone with sgRNA targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQCi-hTRAC-sgRNA

Plasmid

#154093

Purpose

pQCi backbone with sgRNA targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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hTRAF2T117ApLPCX-HA-Flag/P875

Plasmid

#20230

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

T117A (a349g in TRAF2 CDS)

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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hTRAF2T117ApLPCX-HA-Flag/P875

Plasmid

#20230

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

T117A (a349g in TRAF2 CDS)

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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hTRAF2K31RpLPCX-HA-Flag/P876

Plasmid

#20231

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K31R (a92g in the TRAF2 CDS sequence).

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
hTRAF2K31RpLPCX-HA-Flag/P876

Plasmid

#20231

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K31R (a92g in the TRAF2 CDS sequence).

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 A141S

Plasmid

#16157

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

A141S

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 A141S

Plasmid

#16157

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

A141S

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-pnGFP-Ultra

Plasmid

#157923

Purpose

Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra, a high performance peroxynitrite sensor

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-pnGFP-Ultra

Plasmid

#157923

Purpose

Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra, a high performance peroxynitrite sensor

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEBG-GST-TRAF2

Plasmid

#44101

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pEBG-GST-TRAF2

Plasmid

#44101

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
Anti-TRAK2 [N390/43]

Plasmid

#190320

Purpose

Mammalian Expression Plasmid of anti-TRAK2 (Human). Derived from hybridoma N390/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TRAK2 (Homo sapiens) recombinant Mouse monoclonal antibody (TRAK2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TRAK2 [N390/43]

Plasmid

#190320

Purpose

Mammalian Expression Plasmid of anti-TRAK2 (Human). Derived from hybridoma N390/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TRAK2 (Homo sapiens) recombinant Mouse monoclonal antibody (TRAK2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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miR-144 Trap

Plasmid

#60934

Purpose

to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

miR-144 Trap

Use

Lentiviral

Tags

GFP

Promoter

bidirectional phosphoglycerate kinase promoter (b…

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
miR-144 Trap

Plasmid

#60934

Purpose

to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

miR-144 Trap

Use

Lentiviral

Tags

GFP

Promoter

bidirectional phosphoglycerate kinase promoter (b…

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only