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pJMC-L1-Donor-Esp3I

Plasmid

#197842

Purpose

Donor vector to restore L1 destination via Esp3I

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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human K19

Plasmid

#14744

Depositor

M Bishr Omary

Article

Zhou et al J Biol Chem. 1999 Apr 30. 274(18):12861-

Insert

Keratin 19 (KRT19 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_TEV

Plasmid

#58872

Purpose

MESA protease chain with dTomato ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with dTomato ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC-e5

Plasmid

#120343

Purpose

pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

chr8:130594100-130596043 (hg19)

Use

Luciferase

Expression

Mammalian

Promoter

MYC

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC-e4

Plasmid

#120342

Purpose

pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

chr8:129060477-129062593 (hg19)

Use

Luciferase

Expression

Mammalian

Promoter

MYC

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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InterCatch-3xL

Plasmid

#186909

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET1.2-STOP-dsRed

Plasmid

#60944

Purpose

Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

dsRED

Use

Unspecified

Promoter

3XP3

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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tagRFP-GPHN.FingR-PhoCl

Plasmid

#236183

Purpose

Half of photoactivatable GFE3. When co-expressed with PBP-E3-HA (Plasmid #236182), ablates inhibitory synapses after exposure to 400 nm light.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

tagRFP-GPHN.FingR-PhoCl

Expression

Mammalian

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBad-Int Set Flipper

Plasmid

#38209

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Insert

Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

His

Promoter

pBAD/AraC

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0679

Plasmid

#149689

Purpose

Bacterial expression of SENP_EuH protease

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SENP_EuH protease

Tags

His14-tag and Tev protease cleavage site

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.420

Plasmid

#203934

Purpose

Up Flank. Neutral site. Up flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

aquI UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.388

Plasmid

#203940

Purpose

Up Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

mrr UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.389

Plasmid

#203941

Purpose

Down Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

mrr DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Smyd2

Plasmid

#13521

Depositor

Shelley Berger

Article

Huang et al Nature. 2006 Nov 30. 444(7119):629-32.

Insert

Smyd2 (Smyd2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
InterTag-3xL

Plasmid

#186913

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)3-PDGFR

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxKOk

Plasmid

#229949

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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