We narrowed to 59,952 results for: %s

1. pCX-SpiCee-Kras

   Plasmid

   #140838

   Purpose

   mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-Kras
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-Ms-c-Myc-W136E

   Plasmid

   #26024

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc (Myc Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   W136E

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

3. Donor-PIGA

   Plasmid

   #153530

   Purpose

   Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 1,991-bp fragment from intron 5 and exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Mutation

   No mutation

   Promoter

   No promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-FKBP-GFP-NAP1

   Plasmid

   #208862

   Purpose

   Stably express GFP-tagged NAP1 for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

   Plasmid

   #166873

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA array presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SaCas9 TadDE

   Plasmid

   #193846

   Purpose

   Express TadDE (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE with SaCas9
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-Beclin1 1-242

   Plasmid

   #24391

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-242.

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry

   Plasmid

   #192634

   Purpose

   Lentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.

   Depositor

   Yingxiao Wang

   Article

   Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

   Insert

   firefly luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Minimal promoter

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 PARL-FLAG-CT S277G

   Plasmid

   #13615

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed catalytic Serine 277 to Alanine

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

    Plasmid

    #153527

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#68

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#68
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human NANOS3-P2A-mCherry knock-in targeting construct

    Plasmid

    #59721

    Purpose

    Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    P2A mCherry
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-tagRFP-NLS-HA-Bxb1

    Plasmid

    #65625

    Purpose

    Vector for cotranslational expression of tagRFP-Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Bxb1 integrase
    

    Tags

    HA, SV40 NLS, and tagRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMP71-mGFP-LLO-mPMEL

    Plasmid

    #174608

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane GFP fused to LLO and mouse PMEL antigen
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV103

    Plasmid

    #63193

    Purpose

    pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Worm and Yeast

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCBE4-gam

    Plasmid

    #124449

    Purpose

    Expresses dead Cas9 (dCas9)-CBE4-gam

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dCBE4-gam
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-CRY2-mCherry-MYPT169

    Plasmid

    #178526

    Purpose

    Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry-MYPT169
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK1179

    Plasmid

    #179296

    Purpose

    Expresses dCas9 fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-DmrA(x4)
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only