We narrowed to 59,216 results for: %s

1. pEGFP-N1-Barkor

   Plasmid

   #24402

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Barkor (ATG14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pAV11K

   Plasmid

   #63184

   Purpose

   pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFX-β-tubulin-iRFP

   Plasmid

   #175830

   Purpose

   Express iRFP with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with microtuble-targeting sequence
   

   Use

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PliaG

   Plasmid

   #55174

   Purpose

   Constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   LiaG promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSC1-D4Z4-GiP

   Plasmid

   #58542

   Purpose

   Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1-alpha

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

   Plasmid

   #84258

   Purpose

   Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgTRE3G
   PYL1-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mouse U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-Cas12c2-noncoding

   Plasmid

   #120877

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12c2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-POLArISact

   Plasmid

   #164970

   Purpose

   T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   POLArISact
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV114

   Plasmid

   #63181

   Purpose

   pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SNAP-MIDAS

    Plasmid

    #171604

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1121 pcDNA3 GFP FKHR H215R

    Plasmid

    #9027

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pHAGE2-EF1alpha-HA-TFAP4

    Plasmid

    #110158

    Purpose

    Lentiviral vector for expression of human TFAP4 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTYF-super-mfPV-0.8k-Venus-WPRE

    Plasmid

    #156399

    Purpose

    Expresses Venus under PV gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2 Flag Smad3 S204A

    Plasmid

    #27114

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    S204A

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-HA-MEKK1-1-1493

    Plasmid

    #21632

    Depositor

    Melanie Cobb

    Article

    Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

    Insert

    mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIG-Flag-RIPK1-D324A

    Plasmid

    #102678

    Purpose

    retroviral expression plasmid for RIPK1-D324A

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and IRES-GFP

    Expression

    Mammalian

    Mutation

    CHANGE Glutamate 324 to Alanine

    Promoter

    unknown

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-RiboJ-mScarlet-T7

    Plasmid

    #173227

    Purpose

    Encodes mScarlet fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    mScarlet
    

    Use

    Cell-free system

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUPD2 PhiC31 integrase (GB1496)

    Plasmid

    #160578

    Purpose

    Streptomyces phage PhiC31 integrase, complete CDS

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Beclin1 (1-242)

    Plasmid

    #24401

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-242 only.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only