We narrowed to 30,736 results for: genome
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pICH47802-p35S::tdTomato-ER::tNOS
Plasmid#160220PurposetdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverseDepositorInsertp2x35S::TdTomato-ER::tNOS
UseSynthetic Biology; Moclo toolkit level 1 acceptor…ExpressionPlantAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP2A
Plasmid#183324PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP2A geneDepositorInsertTOP2A
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN716 - pCAGGS-eGFP-3Xnls-bpA
Plasmid#156435PurposeFor transient expression of nuclear eGFP in mammalian cellsDepositorInserteGFP
ExpressionMammalianMutationnoneAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdMCP-mCherry
Plasmid#99909PurposeFor the expression of NLS-tdMCP-mCherryDepositorInsertNLS-tdMCP-mCherry
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pSmart-EF1a-Nla-cas5
Plasmid#178878PurposeExpression of human codon-optimized Nlacas5 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas5c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas8
Plasmid#178880PurposeExpression of human codon-optimized NlaCas8 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas8c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas11
Plasmid#178882PurposeExpression of human codon-optimized NlaCas11 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas11c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G13A-tmpknot-epegRNA
Plasmid#214095PurposeLentiviral vector expressing epegRNA to induce KRAS G13A mutationDepositorInsertKRAS G13A epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK61
Plasmid#228121PurposeExpress node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-mB2M
Plasmid#154091PurposeSelf-Cutting and Integrating CRISPR backbone targeting murine B2MDepositorInsertsgRNA and BAIT targeting murine B2M locus
UseCRISPRExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-Nipbl_BSD_FKBPF36V_EYFP
Plasmid#163882PurposeAcute Depletion of NIPBL-EYFPDepositorInsertNipbl (Nipbl Mouse)
ExpressionMammalianAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarca4
Plasmid#122146PurposeFor making Smarca4 RNADepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3006
Plasmid#91096PurposeModule C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoterFMV 34SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdPCP-EGFP
Plasmid#99910PurposeFor the expression of NLS-tPMCP-EGFPDepositorInsertNLS-tdPCP-EGFP
UseLentiviralExpressionMammalianPromoterTRE4GAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
dABE
Plasmid#124447PurposeExpresses dead Cas9 (dCas9)-ABE7.10DepositorInsertdABE7.10
UseCRISPRExpressionMammalianMutationD10A, H840AAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only
