We narrowed to 26,099 results for: lars

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pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX302_circMTO1_OE

Plasmid

#215240

Purpose

Overexpression of pLEX302_circMTO1_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circMTO1

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#232125

Purpose

A middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 / TCF3 (TCF7L1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - H

Plasmid

#229972

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - H

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2AmcherryP2AP -TagBFPH

Plasmid

#229971

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2AmcherryP2AP -TagBFPH

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

Plasmid

#231568

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

All-in-one LOOMINA construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_dCas9-AsLOV2x3-NLSx3

Plasmid

#231567

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_YES_i2

Plasmid

#231419

Purpose

This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-Y

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper TR G414C

Plasmid

#207612

Purpose

U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

Telomerase RNA G414C

Expression

Mammalian

Promoter

U1

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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aTY171_pPGK1_iRFP_WPRE

Plasmid

#225351

Purpose

Lentiviral vector for iRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

iRFP670

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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aTY173_pPGK1_mTagBFP2_WPRE

Plasmid

#225352

Purpose

Lentiviral vector for BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC-MTS

Plasmid

#228827

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

MTS and eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2

Plasmid

#228829

Purpose

Insect cell expression of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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BALF5-D755N-D757N

Plasmid

#192455

Purpose

Expresses BALF5 with D755N and D757N mutations

Depositor

Bill Sugden

Article

Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

Insert

BALF5 (D755N&D757N) (BALF5 Epstein-Barr Virus)

Tags

FLAG

Expression

Mammalian

Mutation

D755N & D757N

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V1

Plasmid

#226538

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V2

Plasmid

#226539

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V3

Plasmid

#226540

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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