We narrowed to 10,128 results for: Met
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Plasmid#17772DepositorPublicationInsertmiR-1003 seed mutant perfect site
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ45 (miR-1003 5' splice site mut)
Plasmid#17775DepositorPublicationInsertdme-miR-1003 minigene 5' splice mut
UseDrosophila metallothionein gene promoter drives e…ExpressionInsectMutation5' splice site mut + compsensatory stemAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)
Plasmid#96888Purposeexpresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)DepositorInsertTrifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
TagsFLAG-taggedExpressionBacterialMutationdeleted amino acid 1-36, mutated lysine 351 to ar…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G596C
Plasmid#131712PurposeMammalian Expression of Flag tagged BRAFDepositorAvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G469S
Plasmid#131716PurposeMammalian Expression of Flag tagged BRAFDepositorAvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-V600R
Plasmid#131721PurposeMammalian Expression of Flag tagged BRAFDepositorAvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro DHFR.dn-cHSF1
Plasmid#134731PurposeLentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.dn-cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202, 379−529PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-myc-BKαM-V5-His
Plasmid#255909PurposeExpress BK channel α subunit's main region (residues 44–651) as a protein module by deletion of N-terminal residues 1–43 and C-terminal residues 652–1113.DepositorInsertKCNMA1 (partial) (KCNMA1 Human)
Tags6xHis and MycExpressionMammalianMutationresidues 44–651 onlyPromoterCMVAvailable sinceJune 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
R929-X81-566
Plasmid#246651PurposeBacterial protein expression of His6-MBP-tev-Hs.HRAS(1-169) G13DDepositorInsertHis6-MBP-tev-Hs.HRAS(1-169) G13D (KRAS Human)
TagsHis6-MBPExpressionBacterialMutationG13DAvailable sinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD allW D262V
Plasmid#234616PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.DepositorInserthnRNPA1_LCD_allW D262V (HNRNPA1 Human)
Tags6xHis-TEVExpressionBacterialMutationC-terminal domain (186-320) bearing familial ALS …PromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD allW D262N
Plasmid#234615PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.DepositorInserthnRNPA1_LCD_allW D262N (HNRNPA1 Human)
Tags6xHis-TEVExpressionBacterialMutationC-terminal domain (186-320) bearing familial ALS …PromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K49R-STAT3
Plasmid#203721PurposeExpresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
TagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K49R substitutionsPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K685R-STAT3
Plasmid#203722PurposeExpresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
TagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K685R substitutionsPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)
Plasmid#180823PurposeExpression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2DepositorAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
Plasmid#175051PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorExpressionBacterialMutationchanged Thr76 to Asp, phosphomimetic mutationPromoterT7 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-6His-mRplp1
Plasmid#173153Purposemouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cellsDepositorInsertORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)
TagsHisExpressionMammalianMutationWild typeAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-gB-BlastR [M1G]
Plasmid#171816PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.DepositorInsertmScarlet-F-gB-T2A-BlastR
UseGene taggingMutationBlastR: Changed Methionin 1 to Glycine.Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPaint-mScarlet-F-gB-PuroR [M1G]
Plasmid#171812PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1DepositorInsertmScarlet-F-gB-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Venus-Bad-2A-pEGFP-C1
Plasmid#166748PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domainDepositorInsertBad-2A (BAD Human)
TagsVenusExpressionMammalianMutation2 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by