Addgene

We narrowed to 3,030 results for: AER

Showing: 81 - 100 of 3030 results

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pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLux and pTet

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pStrepIHFb

Plasmid

#149385

Purpose

Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta

Use

Tags

HRV3C cleavage site and StrepII-tag with

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 shRNA

Plasmid

#122020

Purpose

Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 shRNA

Plasmid

#122023

Purpose

Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pTAMACHis6_PiuD

Plasmid

#128953

Purpose

expresses PiuD in E.coli

Depositor

James Naismith

Article

Luscher et al Antimicrob Agents Chemother. 2018 May 25

Insert

Putative outer membrane receptor for iron transport (PiuD)

Use

Tags

6xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-105)

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Article

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122022

Purpose

Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (AUU…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 WT puroR

Plasmid

#46749

Purpose

Retroviral vector that expresses wild type HA-tagged wild type human USP11

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBBR_Cas9_sp1

Plasmid

#154851

Purpose

Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

Depositor

John van der Oost

Article

Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

Inserts

Spcas9 gene
sgRNA scaffold

Use

CRISPR

Tags

6xHis

Expression

Mutation

Codon harmonized variant for expression in Rhodob…

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 CS puroR

Plasmid

#46750

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pS238D1::tse5-CT

Plasmid

#192958

Purpose

For expression of Tse5-CT (1169-1317) in P. putida for biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 WT puroR

Plasmid

#46753

Purpose

Retroviral vector that expresses wild type HA-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeE

Plasmid

#129120

Purpose

pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

PBAD

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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