We narrowed to 4,686 results for: pcr

1. pCRUSH

   Plasmid

   #54480

   Purpose

   Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro

   Depositor

   Laurie Jackson-Grusby

   Article

   Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and RNAi

   Tags

   SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCri-8a

   Plasmid

   #61317

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCri-4b

   Plasmid

   #61328

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and TRX-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pmSTpCR21

   Plasmid

   #35628

   Purpose

   Bacterial expression of mSERT

   Depositor

   Howard Gu

   Article

   Han et al BMC Pharmacol. 2006 Mar 3;6:6.

   Insert

   SERT (Slc6a4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPR_RAF1_94

   Plasmid

   #111826

   Purpose

   Knockout Raf1

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting RAF1 94-114 (RAF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRRNA-G0-R0

   Plasmid

   #101803

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcrRNA.ind

   Plasmid

   #61284

   Purpose

   Arabinose-inducible repeat-only E. coli CRISPR array

   Depositor

   Chase Beisel

   Article

   Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR2_+1D

    Plasmid

    #149389

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR1272

    Plasmid

    #111107

    Purpose

    Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR1162

    Plasmid

    #111098

    Purpose

    Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCA gRNA (FANCA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcR295

    Plasmid

    #17678

    Depositor

    David Shalloway

    Article

    Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

    Insert

    c-src K295R (SRC Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys 295 changed to Arg.

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCri-4a

    Plasmid

    #61314

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and TRX-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcryBFP_Umyo18bGFP

    Plasmid

    #172527

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

    Depositor

    Joachim Berger

    Article

    Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

    Insert

    myo18b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR1276

    Plasmid

    #111115

    Purpose

    Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51C gRNA (RAD51C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCR1277

    Plasmid

    #111101

    Purpose

    Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51D gRNA (RAD51D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCri-7b

    Plasmid

    #61330

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCR1

    Plasmid

    #154270

    Purpose

    CRISPR-targeted-plasmid

    Depositor

    Dongchang Sun

    Insert

    CR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CCG-AAG

    Promoter

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCri-6a

    Plasmid

    #61315

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    GST-tag and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only