Addgene

We narrowed to 2,012 results for: sp6

Showing: 81 - 100 of 2012 results

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pSP64-AGS-mCherry

Plasmid

#135596

Purpose

Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-SP65

Plasmid

#21089

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Bromo domain aa 1107-1172

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Article

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Tags

Expression

Mutation

T17N. Dominant negative.

Promoter

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
CBP-HAT-SP65

Plasmid

#21088

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

HAT domain aa 1172-1621

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSP64-H2B-GFP

Plasmid

#135605

Purpose

Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-GFP

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CBP-CH3-SP65

Plasmid

#21092

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Expression

Mutation

CH3 domain aa 1625-1877

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSP64T X11 (DM#103)

Plasmid

#15043

Depositor

Douglas Melton

Article

Ku et al Development. 1993 Dec . 119(4):1161-73.

Insert

Xwnt-11 (wnt11b.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64R1 SHP2 D61A

Plasmid

#8326

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Tags

Expression

Mutation

D61A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a F656A

Plasmid

#53054

Purpose

Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

changed Phenylalanine 656 to Alanine

Promoter

SP6

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a Y652A

Plasmid

#53053

Purpose

Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

changed Tyrosine 652 to Alanine

Promoter

SP6

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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P29-sp64 Poly A

Plasmid

#59434

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 29

Use

Makes synthetic transcripts that contains poly a

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSP64R1 SHP2 E76A

Plasmid

#8327

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Tags

Expression

Mutation

E76A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
P21-sp64 Poly A

Plasmid

#59433

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 21

Use

Makes synthetic transcripts that contains poly a

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
HOXB4-FLAG-WG-SP65

Plasmid

#21087

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

Insert

HOXB4 (HOXB4 Human)

Use

In vitro t/t

Tags

Expression

Mutation

W to G mutation in YPWM Pbx1 interaction motif, p…

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pSP64T BMPR (DM#136)

Plasmid

#15068

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
ADEPT-pTarget-Msp6

Plasmid

#238039

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

lac

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA:NbCysP6

Plasmid

#223217

Purpose

Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

Depositor

Tsepo Tsekoa

Article

Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

Insert

NbCysP6 sgRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Plant

Mutation

Promoter

Arabidopsis U6 promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-sp6-kcnk4b

Plasmid

#213123

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk4b (LOC101882857 Zebrafish)

Use

Cloning

Tags

Expression

Mutation

Stop codon removed

Promoter

T7

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp6

Plasmid

#168955

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp6 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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