We narrowed to 4,244 results for: Pet2

1. pET-MBP-creM

   Plasmid

   #112238

   Purpose

   pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

   Depositor

   Emily Balskus

   Article

   Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

   Insert

   CreM
   

   Use

   Tags

   MBP

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 9AVA

   Plasmid

   #220884

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. KE-009

   Plasmid

   #133441

   Purpose

   Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4H (EIF4H Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RG-001

   Plasmid

   #133439

   Purpose

   Heat 3 domain of human elF4G in pET21 backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4G1 (EIF4G1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 8VL7

   Plasmid

   #220883

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pAM75

   Plasmid

   #224249

   Purpose

   pET28a MWG-ubiquitin-G77-C78

   Depositor

   Andreas Martin

   Article

   Xie et al Methods Mol Biol. 2023;2591:79-100. doi:

   Insert

   MWG-ubiquitin-G77-C78
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-MBP-CreM_E352A

   Plasmid

   #112239

   Purpose

   pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM with E352A mutation

   Depositor

   Emily Balskus

   Article

   Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

   Insert

   CreM_E352A
   

   Use

   Tags

   MBP

   Expression

   Mutation

   Glutamate 352 (E352) mutated to an alanine.

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PUF Assembly Kit

   Plasmid Kit

   #1000000051

   Purpose

   Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.

   Depositor

   Huimin Zhao

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLZL2372

   Plasmid

   #48683

   Purpose

   pET29a_HHH CODA HIV integrase with C-terminal HIS tag

   Depositor

   Suzanne Sandmeyer

   Article

   Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

   Insert

   HIV Integrase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLZL2374

    Plasmid

    #48684

    Purpose

    pET29a_PPP CODA Ty3 integrase with C-terminal His tag

    Depositor

    Suzanne Sandmeyer

    Article

    Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

    Insert

    Ty3 Integrase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLZL2386

    Plasmid

    #48682

    Purpose

    pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

    Depositor

    Suzanne Sandmeyer

    Article

    Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

    Insert

    PFV Integrase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pH6-mNeon

    Plasmid

    #178460

    Purpose

    The mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-28a mScarlet

    Plasmid

    #178543

    Purpose

    The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mScarlet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAM76

    Plasmid

    #227625

    Purpose

    pET28a Ubiquitin-G77C78

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Insert

    Ub (UBI4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G77C78

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH6-2xmNeon

    Plasmid

    #178462

    Purpose

    A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen dimer
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTorD-H6

    Plasmid

    #169039

    Purpose

    Escherichia coli TorD with C-terminal 6xHis in pET28a.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    TorD-H6
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    The mutation N at position 46 was changed back to…

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW016

    Plasmid

    #131820

    Purpose

    pET28b derrivative vector with T7-driven IPTG inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HSA3_His

    Plasmid

    #202765

    Purpose

    human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA3
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BSA1_His

    Plasmid

    #202766

    Purpose

    bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    BSA1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HSA1_His

    Plasmid

    #202763

    Purpose

    human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HSA2_His

    Plasmid

    #202764

    Purpose

    human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pH6-spTorA-mCherry

    Plasmid

    #169040

    Purpose

    mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-mCherry
    

    Use

    Tags

    6xHis and spTorA (signal peptide of E. coli TorA)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BSA2_His

    Plasmid

    #202767

    Purpose

    bovine serum albumin design BSA2, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    BSA2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BSA3_His

    Plasmid

    #202768

    Purpose

    bovine serum albumin design BSA3, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    BSA1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pH6-mNeonC

    Plasmid

    #178461

    Purpose

    The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Free cysteine at the C-terminus

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pH6-2xmNeonC

    Plasmid

    #178463

    Purpose

    A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen dimer
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Free cysteine at the C-terminus of the dimer

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Traptavidin-E6

    Plasmid

    #59549

    Purpose

    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Core Traptavidin-E6
    

    Use

    Tags

    E6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

28. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSNRW-Z3-Nluc

    Plasmid

    #174486

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z3-Nluc
    

    Use

    Tags

    6x Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p-preSufI(IAC)

    Plasmid

    #168516

    Purpose

    Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    pre-SufI(IAC)
    

    Use

    Tags

    6xHisC tag and HSV tag

    Expression

    Bacterial

    Mutation

    C17I and C295A

    Promoter

    T7

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAM232

    Plasmid

    #226654

    Purpose

    pET24b-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Dead Streptavidin-SpyTag

    Plasmid

    #59548

    Purpose

    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead Streptavidin-SpyTag
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    The construct lacks final K of SpyTag

    Promoter

    T7

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pAM221

    Plasmid

    #226645

    Purpose

    pET24b-p97-R191Q-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R191Q

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAM224

    Plasmid

    #226647

    Purpose

    pET24b-p97-T262A-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    T262A

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAM220

    Plasmid

    #226644

    Purpose

    pET24b-p97-R159H-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R159H

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAM219

    Plasmid

    #226643

    Purpose

    pET24b-p97-R155H-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R155H

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAM222

    Plasmid

    #226646

    Purpose

    pET24b-p97-L198W-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L198W

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge