We narrowed to 1,046 results for: Psp;

1. pCPH2A.X_K5R-GS3

   Plasmid

   #204747

   Purpose

   Expression of Cas9 and human H2AX with the K5R mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K5R (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the K5R mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPH2A.X_K134L-GS3

   Plasmid

   #204750

   Purpose

   Expression of Cas9 and human H2AX with the K134L mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134L (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the K134L mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCPH2A.X_K134M-GS3

   Plasmid

   #204751

   Purpose

   Expression of Cas9 and human H2AX with the K134M mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134M (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the K134M mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPH2A.X_K134A-GS3

   Plasmid

   #204752

   Purpose

   Expression of Cas9 and human H2AX with the K134A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134A (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the K134A mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. Px461-Cas9n-Trp53-sgRNA-alpha

   Plasmid

   #88846

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDG462

   Plasmid

   #100903

   Purpose

   SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RBM14_pLX307

   Plasmid

   #98367

   Purpose

   Lentiviral expression of RBM14

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   RBM14 (RBM14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPE

    Plasmid

    #15284

    Depositor

    Ron Vale

    Article

    Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

    Insert

    ubiquitous kinesin heavy chain (KIF5B Human)
    

    Expression

    Bacterial

    Mutation

    The insert is missing the 1st 180 bp of 5' u…

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

14. sgAAVS1(A)

    Plasmid

    #85570

    Purpose

    Expresses sgRNA targeting human AAVS1 locus

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    adeno-associated virus integration site 1 (AAVS1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

15. sgAAVS1(B)

    Plasmid

    #85571

    Purpose

    Expresses sgRNA targeting human AAVS1 locus

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    adeno-associated virus integration site 1 (AAVS1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

16. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

    Plasmid

    #91882

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

17. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

18. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6F

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

19. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. INS-3'gRNA

    Plasmid

    #210468

    Purpose

    gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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