We narrowed to 2,403 results for: T2A

1. EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

   Plasmid

   #194232

   Purpose

   Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   EF1a-LCB3-PDGFR TMD-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

   Plasmid

   #194234

   Purpose

   Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

   Plasmid

   #223504

   Purpose

   Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

   Depositor

   James Frank

   Insert

   dead-mutant SNAP-tag and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SNAP-tag C145A

   Promoter

   hSyn

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-Sox17-p2A-Erg-t2A-GFP

   Plasmid

   #222496

   Purpose

   mouse fibroblast-to-endothelial cell direct reprogramming

   Depositor

   Li Qian

   Article

   Farber et al Nat Commun. 2024 May 16;15(1):4170. doi:

   Inserts

   Sox17 (Sox17 Mouse)
   Erg (Erg )
   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Puro cTnT CCND2-T2A-ChloC

   Plasmid

   #179841

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   chloride-conducting ChR (ChloC)

   Expression

   Mammalian

   Mutation

   Promoter

   Troponin T promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-Puro cTnT CCND2-T2A-iChloC

   Plasmid

   #179842

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   improved chloride-conducting ChR (iChloC)

   Expression

   Mammalian

   Mutation

   Promoter

   Troponin T promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Puro cTnT CCND2-T2A-luciferase

   Plasmid

   #179844

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR, Luciferase, and TALEN ; Donor plasmid

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Troponin T promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)

   Plasmid

   #221330

   Purpose

   Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   FLAG-IRSp53 and HA-Rac1(G12V) (Baiap2, Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   TRE3G

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p186-RV-CAG-dio-mScarlet-T2A-mVenus

   Plasmid

   #204740

   Purpose

   Conditional expression of target gene mVenus with fluorescent reporter mScarlet

   Depositor

   Lynette Lim

   Article

   Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

   Insert

   mVenus
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

    Plasmid

    #195501

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4

    Plasmid

    #193295

    Purpose

    episomal vector expressing mCherry + human SOX2A61V + KLF4

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2AV-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid

    #141100

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJET-63-SFFV-T2A-BlaR-polyA-R11a

    Plasmid

    #179894

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJET-61-SFFV-T2A-PuroR-polyA-R11a

    Plasmid

    #179892

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid

    #179893

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid

    #179895

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid

    #182362

    Purpose

    Shuttle vector for T2A-Myr-Casp8-ERT2

    Depositor

    Mario Capecchi

    Insert

    loxP-T2A-Myr-Casp8-ERT2-rox
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. loxP-T2A-Myr-DeadCasp8-ERT2-rox

    Plasmid

    #182361

    Purpose

    Shuttle vector for T2A-Myr-DeadCasp8-ERT2

    Depositor

    Mario Capecchi

    Insert

    loxP-T2A-Myr-DeadCasp8-ERT2-rox
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mT2A-TagRFP-T-linker-dre-PBD*-FNF

    Plasmid

    #182342

    Purpose

    Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-TagRFP-T-linker-dre-PBD*-FNF
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-hGFAP-ND1-K139D-T2A-mCherry

    Plasmid

    #178587

    Purpose

    Expression of NEUROD1-K139D and mCherry under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1-K139D (NEUROD1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NEUROD1-K139D

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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