We narrowed to 7,391 results for: ef1a;

1. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

   Plasmid

   #100941

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
   

   Use

   Tags

   1xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-UbC-FKRP-EF1a-BSD

   Plasmid

   #205150

   Purpose

   Express UbC-driven FKRP and EF-1a driven BSD

   Depositor

   Monkol Lek

   Insert

   Fukutin-related protein (FKRP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

   Plasmid

   #201032

   Purpose

   Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Eef1a-1955/-1>nls::Cas9::nls

   Plasmid

   #59987

   Purpose

   Eef1a (EF1alpha) promoter driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Eef1a (EF1alpha) -1955/-1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

   Plasmid

   #189807

   Purpose

   Retroviral delivery of Cas9

   Depositor

   Ellen Rothenberg

   Article

   Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-tdTomato-WPRE

   Plasmid

   #133786

   Purpose

   Cre-dependent tdTomato fluorescent reporter for cell labeling

   Depositor

   Michael Stryker

   Article

   Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

   Insert

   pAAV-EF1a-DIO-tdTomato-WPRE
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF0690 Ef1a-Kp03_attB-BFP acceptor

    Plasmid

    #193473

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-ASAP3-WPRE

    Plasmid

    #132318

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid

    #35498

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-ConVERGD-eGFP-W3SL

    Plasmid

    #218744

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lv EF1a SS18-Frb PGK Puro

    Plasmid

    #102811

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    SS18-Frb
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1a-hDNAJC6-T2A-copGFP

    Plasmid

    #170443

    Purpose

    Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human DNAJC6 (DNAJC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

    Plasmid

    #198515

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-DIO-JEDI-1P-WPRE

    Plasmid

    #202611

    Purpose

    Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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