Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 18,554 results for: multi

Clear keywords
Showing: 981 - 1000 of 18554 results

  3. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Publication
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    163148-plasmid-map-sequence-id-331409

    Enlarge

  5. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84239-plasmid-map-sequence-id-160811

    Enlarge

  7. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52054-plasmid-map-sequence-id-438919

    Enlarge

  9. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Publication
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

    Enlarge

  11. pcDNA3-RAB-EKARev

    Plasmid
    #118474
    Purpose
    ddRFP-based single-color biosensor for monitoring ERK activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    118474-plasmid-map-sequence-id-243124

    Enlarge

  19. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid
    #103866
    Purpose
    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103866-plasmid-map-sequence-id-199147

    Enlarge

  20. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Expression
    Mammalian
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124230-plasmid-map-sequence-id-240604

    Enlarge

Showing: 981 - 1000 of 18554 results