We narrowed to 8,876 results for: sgrna

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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 2

Plasmid

#131346

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-PP7-boxB

Plasmid

#75393

Purpose

sgRNA1-PP7-boxB

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7-boxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L5L2

Plasmid

#113738

Purpose

Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Expression scaffold for dCas9+sgRNA

Plasmid

#171799

Purpose

PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Insert

PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression

Use

CRISPR

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-R4R3

Plasmid

#113739

Purpose

Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L5L4

Plasmid

#113741

Purpose

Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1R5

Plasmid

#113737

Purpose

Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-RPB1-N-term

Plasmid

#195111

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Tet-inducible Luciferase reporter

Plasmid

#64161

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for Tet-inducible Luciferase reporter

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato-TSC2-sgRNA

Plasmid

#196195

Purpose

Editing human TSC2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

TSC2 (TSC2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2NT

Plasmid

#62257

Purpose

expression of A2NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-boxB-MS2

Plasmid

#75396

Purpose

sgRNA1-boxB-MS2

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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SpyCas9 EMX1-nicking sgRNA

Plasmid

#169859

Purpose

SpyCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpyCas9 EMX1-nicking sgRNA

Expression

Mammalian

Promoter

human U6 promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-2-RPB1-C-term

Plasmid

#195109

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against last exon of RPB1 (C-terminal)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2A-sgRNA

Plasmid

#182551

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2A-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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